Abstract: The study was conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures, and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals, and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants are contributors to the pharmaceutical, agricultural, and food industries in the world. The use of medicinal plants in industrialized societies has been traced to the extraction and development of several drugs used in order to heal some diseases having inhibiting effects against pathogenic microorganisms. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for antimicrobial susceptibility tests. Methodology: The extraction and identification of some phytochemicals crude compounds used for antimicrobial susceptibility test from plant samples such as Ocimum lamiifolium (OL), Croton maerosth (Cm), and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortar and pistil, and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compounds. Result: The main result of the Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to manage numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role in reducing life-threatening ailments of people and other animals.
Firew, Admasu Hailu; Israel, Petros; and Abdurezak, Murad
"Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test,"
Health Sciences: Vol. 1:
1, Article 3.
Available at: https://doi.org/10.15342/hs.2020.236
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.